6N29
Crystal structure of monomeric von Willebrand Factor D`D3 assembly
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 174.690, 174.690, 104.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.450 - 2.500 |
| R-factor | 0.2033 |
| Rwork | 0.202 |
| R-free | 0.24150 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.707 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 5.190 | |
| Number of reflections | 55726 | 3570 |
| <I/σ(I)> | 13.3 | 0.41 |
| Completeness [%] | 98.7 | 87.2 |
| Redundancy | 14.1 | 10.9 |
| CC(1/2) | 0.114 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 297 | 5.5% (w/v) PEG8000, 10% (w/v) PEG1000, 0.2 M calcium acetate and 0.1 M Tris-acetate, pH 8.5 |
