6N1D
X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-03 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.03315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 207.450, 442.870, 613.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 3.200 |
| R-factor | 0.271 |
| Rwork | 0.268 |
| R-free | 0.32100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v67 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmeas | 0.309 | |
| Number of reflections | 874708 | |
| <I/σ(I)> | 3.94 | 0.82 |
| Completeness [%] | 95.5 | 95 |
| Redundancy | 2.87 | 2.49 |
| CC(1/2) | 0.987 | 0.182 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 7 | 295 | pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
