6MYT
Avian mitochondrial complex II with Atpenin A5 bound, sidechain in pocket
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.992, 83.817, 290.344 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.190 - 2.270 |
R-factor | 0.227 |
Rwork | 0.226 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yq3 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.533 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX ((DEV_3150: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.230 | 2.340 |
High resolution limit [Å] | 2.270 | 2.270 |
Rmerge | 0.145 | 0.557 |
Number of reflections | 70243 | |
<I/σ(I)> | 4.1 | 1.5 |
Completeness [%] | 87.6 | 67.8 |
Redundancy | 2.15 | 1.53 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 47 g/L PEG3350, 30 mL/L PEG400, 23 mL/L isopropanol, 0.05 M HEPES sodium, 0.01 M Tris-HCl, 3.1 mM manganese chloride, 0.62 mM magnesium chloride, 1.5 mM sodium azide, 0.25 mM sodium EDTA, 10 g/L octyl beta-D-glucoside, undecyl-beta-D-maltoside, crystal was soaked with Atpenin A5 after crystallization |