6MYR
Avian mitochondrial complex II with thiapronil bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.361, 84.622, 293.337 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.930 - 2.150 |
R-factor | 0.2187 |
Rwork | 0.218 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yq3 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.721 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_3150) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.520 | 2.210 |
High resolution limit [Å] | 2.145 | 2.150 |
Rmerge | 0.108 | 0.604 |
Number of reflections | 89869 | 3430 |
<I/σ(I)> | 4.6 | 1.1 |
Completeness [%] | 90.2 | 42 |
Redundancy | 3.1 | 2.21 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 47 g/L PEG3350, 30 mL/L PEG400, 23 mL/L isopropanol, 0.05 M HEPES sodium, 0.01 M Tris-HCl, 3.1 mM manganese chloride, 0.62 mM magnesium chloride, 1.5 mM sodium azide, 0.25 mM sodium EDTA, 10 g/L octyl beta-D-glucoside, undecyl-beta-D-maltoside, crystal was soaked with thiapronil after crystallization |