6MYJ
Pleurotus ostreatus OstreolysinA plus sphingomyelin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-10 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97926 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.425, 100.565, 58.813 |
Unit cell angles | 90.00, 106.29, 90.00 |
Refinement procedure
Resolution | 44.561 - 1.330 |
R-factor | 0.1548 |
Rwork | 0.155 |
R-free | 0.16750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ov8 |
RMSD bond length | 0.016 |
RMSD bond angle | 0.949 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.350 |
High resolution limit [Å] | 1.330 | 3.610 | 1.330 |
Rmerge | 0.046 | 0.028 | 0.429 |
Rmeas | 0.049 | 0.030 | 0.462 |
Rpim | 0.018 | 0.011 | 0.170 |
Number of reflections | 117866 | 6004 | 5878 |
<I/σ(I)> | 8 | ||
Completeness [%] | 99.4 | 99.5 | 99.7 |
Redundancy | 7.5 | 7.7 | 7.2 |
CC(1/2) | 0.999 | 0.906 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M ammonium chloride, 0.05 M tris hydrochloride, 0.15 M sodium chloride, 19% (w/v) PEG3350, saturated sphingomyelin, saturated cholesterol, 30% ethylene glycol |