6MXC
Crystal structure of Trypanosoma brucei hypoxanthine-guanine-xanthine phosphoribosyltranferase in complex with GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-06 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.021, 107.321, 117.214 |
| Unit cell angles | 90.00, 96.93, 90.00 |
Refinement procedure
| Resolution | 44.744 - 1.993 |
| R-factor | 0.1533 |
| Rwork | 0.152 |
| R-free | 0.19970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6AP2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.151 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.744 | 2.030 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.088 | |
| Rpim | 0.061 | 0.213 |
| Number of reflections | 102156 | 4961 |
| <I/σ(I)> | 11.3 | 3 |
| Completeness [%] | 99.9 | 98.7 |
| Redundancy | 5.7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293.15 | 25% PEG 3350, 0.2 M lithium sulphate and 0.1 M Bis-Tris pH 5 |
