6MVX

NavAb Voltage-gated Sodium Channel, I217C, in Complex with Class 1C Anti-arrhythmic Flecainide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-18
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	177.525, 177.687, 192.923
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.855 - 3.455
R-factor	0.236
Rwork	0.230
R-free	0.26290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rvy tetramer
RMSD bond length	0.004
RMSD bond angle	0.670
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.579
High resolution limit [Å]	3.455	3.455
Rmerge	0.157
Number of reflections	39868
<I/σ(I)>	8.75
Completeness [%]	99.9
Redundancy	6.3
CC(1/2)		0.431

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	1.8 M Ammonium Sulfate 100 mM Sodium Acetate, pH 5.0 100 uM Flecainide