6MVW
NavAb voltage-gated sodium channel, I217C/F203W
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 126.220, 126.090, 192.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.963 - 3.198 |
| R-factor | 0.2175 |
| Rwork | 0.212 |
| R-free | 0.24590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rvy tetramer |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.544 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.312 |
| High resolution limit [Å] | 3.198 | 3.198 |
| Rmerge | 0.186 | |
| Number of reflections | 51427 | |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 7.3 | |
| CC(1/2) | 0.283 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 1.8 M Ammonium Sulfate, 100 mM Sodium Acetate |
