6MV7
Crystal structure of RNAse 6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-04 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 27.650, 38.830, 97.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.095 - 2.590 |
R-factor | 0.2204 |
Rwork | 0.217 |
R-free | 0.25190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mv6 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.703 |
Data reduction software | HKL-2000 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.100 | 2.710 |
High resolution limit [Å] | 2.590 | 2.600 |
Number of reflections | 3537 | 308 |
<I/σ(I)> | 17.2 | |
Completeness [%] | 98.6 | |
Redundancy | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 298 | PEG 4000, 50mM Sodium phosphate |