6MUF

Crystal Structure of HIV-1 B41 SOSIP.664 Prefusion Env Trimer in Complex with Human Antibodies 3H109L and 35O22 at 3.4 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-05
Detector	RAYONIX MX300-HS
Wavelength(s)	1
Spacegroup name	P 63
Unit cell lengths	131.970, 131.970, 314.690
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.836 - 2.910
R-factor	0.2549
Rwork	0.253
R-free	0.29700
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.950
High resolution limit [Å]	2.900	7.860	2.900
Rmerge	0.076	0.037	1.079
Rmeas	0.087	0.043	1.184
Rpim	0.041	0.021	0.483
Total number of observations	96862
Number of reflections	29704	3074	147
<I/σ(I)>	6.9
Completeness [%]	43.9	89.2	4.4
Redundancy	3.3	2.7	5.8
CC(1/2)		0.992	0.537

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		298	90mM Na Acetate pH5.5 6% PEG8,000 3% PEG400 450mM NaCl