6MUD
Voltage-gated sodium channel NaV1.5 C-terminal domain in complex with Ca2+/Calmodulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.033000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 65.579, 84.032, 140.684 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.310 - 2.690 |
R-factor | 0.2147 |
Rwork | 0.212 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.055 |
Data reduction software | XDS (November 11, 2013) |
Data scaling software | XDS (November 11, 2013) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.850 |
High resolution limit [Å] | 2.690 | 2.690 |
Rmeas | 0.097 | 0.494 |
Number of reflections | 10476 | 1471 |
<I/σ(I)> | 9.51 | 1.89 |
Completeness [%] | 93.9 | 83.3 |
Redundancy | 3.25 | 2.39 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 277 | 5-15 % (w/v) PEG 4000, 0.1 M Tris, pH 9.5, 0.1 M MgCl2, and 5 % (v/v) isopropanol |