6MRD
ADP-bound human mitochondrial Hsp60-Hsp10 half-football complex
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.82 |
| Software | RELION,cryoSPARC,Leginon,CTFFIND,Coot,PHENIX |
| Symmetry | C7 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
