6MR4
Crystal structure of the Sth1 bromodomain from S.cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 141.753, 74.619, 72.541 |
Unit cell angles | 90.00, 92.88, 90.00 |
Refinement procedure
Resolution | 72.449 - 2.710 |
R-factor | 0.2394 |
Rwork | 0.238 |
R-free | 0.26990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tlp |
RMSD bond length | 0.006 |
RMSD bond angle | 0.610 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.450 | 2.840 |
High resolution limit [Å] | 2.710 | 2.710 |
Rmerge | 0.083 | |
Number of reflections | 20385 | 2722 |
<I/σ(I)> | 10.8 | 1.7 |
Completeness [%] | 98.6 | 99.5 |
Redundancy | 3.1 | 3.2 |
CC(1/2) | 0.990 | 0.600 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M magnesium chloride hexahydrate 0.1 M BIS-TRIS pH 6.5 25% (w/v) Polyethylene glycol 3350 3% Trimethylamine N-oxide dihydrate |