6MQC

Vaccine-elicited NHP FP-targeting neutralizing antibody 0PV-c.01 in complex with FP (residue 512-519)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	73.122, 72.898, 169.305
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.625 - 1.990
R-factor	0.2005
Rwork	0.199
R-free	0.23370
RMSD bond length	0.006
RMSD bond angle	1.053
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.111	0.064	0.808
Rmeas	0.122	0.071	0.896
Rpim	0.050	0.030	0.381
Total number of observations	358892
Number of reflections	62096	3358	3105
<I/σ(I)>	6.1
Completeness [%]	99.6	99.2	99.9
Redundancy	5.8	5.3	5.1
CC(1/2)		0.995	0.792

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M NaNO3 and 20% w/v PEG 3350