6MPI

Structure of the Thermus thermophilus 30S ribosomal subunit complexed with a 2-thiocytidine (s2C32) and inosine (I34) modified anticodon stem loop (ASL) of Escherichia coli transfer RNA Arginine 1 (TRNAARG1) bound to an mRNA with an CGU-codon in the A-site and paromomycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	277
Detector technology	CCD
Collection date	2010-08-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97
Spacegroup name	P 41 21 2
Unit cell lengths	403.960, 403.960, 176.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.766 - 3.330
R-factor	0.1907
Rwork	0.190
R-free	0.21650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xnr
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.8.4_1496)
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.766	3.450
High resolution limit [Å]	3.330	3.330
Number of reflections	354511
<I/σ(I)>	10.83
Completeness [%]	96.4
Redundancy	2.5
CC(1/2)	0.990	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	MPD, NH4Cl, KCl, CaCl2, magnesium acetate, sodium cacodylate, pH 6.5 VAPOR DIFFUSION, HANGING DROP, temperature 277K