6MOK

Dimeric DARPin A_distance_R7 complex with EpoR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-19
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00001
Spacegroup name	P 62 2 2
Unit cell lengths	117.090, 117.090, 233.587
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.790 - 5.101
R-factor	0.23254
Rwork	0.225
R-free	0.29890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	predicted model 1ern
RMSD bond length	0.006
RMSD bond angle	1.443
Data reduction software	XDS (Jan 26, 2018)
Data scaling software	XDS (Jan 26, 2018)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.790	5.283
High resolution limit [Å]	5.101	5.101
Rmerge	0.116	2.750
Rmeas	0.123
Rpim	0.039	0.884
Number of reflections	4205	395
<I/σ(I)>	9.92	0.9
Completeness [%]	98.4	98.74
Redundancy	9.8	10.4
CC(1/2)	0.996	0.585

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	295	0.2 M NaCl, 0.1 M Tris pH 8.5, 25% PEG 3350