6ML8
Crystal structure of hemagglutinin from H1N1 Influenza A virus A/Denver/57 bound to the C05 antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-07-26 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 118.460, 118.460, 162.440 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.886 | 47.886 | 3.000 |
High resolution limit [Å] | 2.920 | 13.060 | 2.920 |
Rmerge | 0.107 | 0.024 | 0.977 |
Rmeas | 0.116 | 0.026 | 1.050 |
Number of reflections | 29212 | 368 | 2117 |
<I/σ(I)> | 15.23 | 43.86 | 2 |
Completeness [%] | 99.9 | 94.1 | 100 |
Redundancy | 7.324 | 5.734 | 7.556 |
CC(1/2) | 0.998 | 0.999 | 0.726 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 287 | InvbJ.18715.a.KN11/HosaA.20194.a.TC11 batch PD39296/PD38299 at a combined complex 10 mg/mL against PACT screen condition G9: 0.2 M potassium/sodium tartrate, 0.1 M BisTris Propane pH 7.5, 20% PEG 3350 supplemented with 20% ethylene glycol as cryo-protectant, crystal tracking ID 300847g9, unique puck ID sto6-4 |