6MIB
Crystal structure of the ILK ATP-binding deficient mutant (L207W)/alpha-parvin core complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-17 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.714, 117.635, 47.775 |
| Unit cell angles | 90.00, 114.54, 90.00 |
Refinement procedure
| Resolution | 20.880 - 1.800 |
| R-factor | 0.16259 |
| Rwork | 0.160 |
| R-free | 0.20778 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.948 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.463 | |
| Number of reflections | 39797 | 1954 |
| <I/σ(I)> | 41.78 | 7.69 |
| Completeness [%] | 98.9 | 95.9 |
| Redundancy | 4.6 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | 16% PEG 3,350, 0.1 M Bis-Tris Propane, pH 7.4, 5% 1-Propanol |
