6MHQ
Structure of connexin-46 intercellular gap junction channel at 3.4 angstrom resolution by cryoEM
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.4 |
Number of Particle | 398066 |
Software | DoG Picker,RELION,PHENIX,SerialEM,Gctf,Coot |
Symmetry | D6 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |