6MGO
Structure of rabbit actin in complex with Mycalolide B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-08 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.744, 75.566, 67.523 |
| Unit cell angles | 90.00, 99.60, 90.00 |
Refinement procedure
| Resolution | 29.570 - 2.200 |
| R-factor | 0.1643 |
| Rwork | 0.163 |
| R-free | 0.19520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s22 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.790 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.085 | 0.352 |
| Rmeas | 0.240 | |
| Rpim | 0.055 | |
| Number of reflections | 20664 | |
| Redundancy | 3.2 | 3.1 |
| CC(1/2) | 0.945 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG 8000, magnesium acetate, cacodylate |
