6MGM
Helix-Loop-helix motif of mouse DNA-binding protein inhibitor ID-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-03-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.95 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.165, 28.979, 50.333 |
Unit cell angles | 90.00, 97.94, 90.00 |
Refinement procedure
Resolution | 21.437 - 1.791 |
R-factor | 0.1832 |
Rwork | 0.178 |
R-free | 0.22270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mdy |
RMSD bond length | 0.007 |
RMSD bond angle | 0.727 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rpim | 0.044 | 0.231 |
Number of reflections | 8782 | |
<I/σ(I)> | 32.8 | |
Completeness [%] | 99.3 | |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1 M sodium citrate (pH 6.5), 0.2 M magnesium acetate, 10% PEG8000 |