6MG1
C-terminal bZIP domain of human C/EBPbeta with 16bp Methylated Oligonucleotide Containing Consensus Recognition Sequence-C2 Crystal Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-04 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 48.110, 77.405, 99.825 |
| Unit cell angles | 90.00, 103.90, 90.00 |
Refinement procedure
| Resolution | 34.542 - 1.750 |
| R-factor | 0.2156 |
| Rwork | 0.214 |
| R-free | 0.24960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e42 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.996 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.542 | 1.690 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.080 | 0.519 |
| Rpim | 0.024 | 0.374 |
| Number of reflections | 35611 | 842 |
| <I/σ(I)> | 25.4 | 1.2 |
| Completeness [%] | 80.8 | |
| Redundancy | 10.3 | |
| CC(1/2) | 0.787 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | 0.2 M sodium chloride, 0.1 M BIS-TRIS pH 6.5, 25% (w/v) polyethylene glycol 3350 |
