6MG0
Crystal structure of a 5-domain construct of LgrA in the thiolation state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 161.549, 212.894, 253.127 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 78.440 - 6.000 |
| R-factor | 0.2468 |
| Rwork | 0.244 |
| R-free | 0.29360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mfw |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.601 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_3092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.440 | 6.480 |
| High resolution limit [Å] | 6.000 | 6.000 |
| Number of reflections | 22451 | 4552 |
| <I/σ(I)> | 6.9 | 1.6 |
| Completeness [%] | 99.8 | |
| Redundancy | 6.3 | |
| CC(1/2) | 0.451 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 277 | 0.25 M sodium fluoride, 3.1 M sodium formate, pH 6.8 |
