6MFX
Crystal structure of a 4-domain construct of a mutant of LgrA in the substrate donation state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-02 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 |
Unit cell lengths | 67.449, 73.987, 77.076 |
Unit cell angles | 94.31, 114.86, 92.23 |
Refinement procedure
Resolution | 69.580 - 2.200 |
R-factor | 0.1963 |
Rwork | 0.195 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mfw |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | PHENIX (dev_3584) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.580 | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 66903 | 4469 |
<I/σ(I)> | 9.1 | 1.6 |
Completeness [%] | 98.0 | 96.7 |
Redundancy | 3.3 | |
CC(1/2) | 0.991 | 0.388 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.1 | 295 | 26% PEG4000, 0.1 M Tris-HCl, pH 8.1 |