6MF6
Crystal structure of budding yeast Cdc5 polo-box domain in complex with the Dbf4 polo-interacting region.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-26 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 65 |
Unit cell lengths | 135.054, 135.054, 75.163 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.207 - 3.400 |
R-factor | 0.2454 |
Rwork | 0.243 |
R-free | 0.28300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1q4o |
RMSD bond length | 0.004 |
RMSD bond angle | 0.711 |
Data reduction software | MOSFLM |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.500 | 3.490 |
High resolution limit [Å] | 3.400 | 3.400 |
Number of reflections | 10923 | |
<I/σ(I)> | 10.92 | |
Completeness [%] | 100.0 | |
Redundancy | 18.9 | |
CC(1/2) | 0.998 | 0.315 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 200 mM sodium potassium tartrate, 10 mM trimethylamine hydrochloride, 20 % PEG 3350. |