6MEI
Crystal structure of broadly neutralizing antibody HEPC3 in complex with Hepatitis C virus envelope glycoprotein E2 ectodomain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.810, 75.790, 173.999 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 86.999 - 2.900 |
R-factor | 0.2112 |
Rwork | 0.208 |
R-free | 0.27690 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 87.000 | 87.000 | 2.270 |
High resolution limit [Å] | 2.200 | 9.070 | 2.200 |
Rmerge | 0.271 | 0.092 | 0.663 |
Rmeas | 0.310 | 0.105 | 0.819 |
Rpim | 0.145 | 0.049 | 0.467 |
Total number of observations | 231097 | 3599 | 15221 |
Number of reflections | 44996 | 761 | 3808 |
<I/σ(I)> | 3.7 | 8.3 | 0.3 |
Completeness [%] | 99.8 | 100 | 98.7 |
Redundancy | 5.1 | 4.7 | 4 |
CC(1/2) | 0.885 | 0.976 | 0.660 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2M sodium bromide, 20% PEG 3,350 |