6MEG
Crystal structure of human monoclonal antibody HEPC46
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.640, 71.302, 108.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.872 - 1.410 |
| R-factor | 0.1756 |
| Rwork | 0.174 |
| R-free | 0.20010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3p30 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.041 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.7.1) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.810 | 48.810 | 1.430 |
| High resolution limit [Å] | 1.410 | 7.720 | 1.410 |
| Rmerge | 0.064 | 0.040 | 0.893 |
| Rmeas | 0.071 | 0.044 | 0.995 |
| Rpim | 0.031 | 0.018 | 0.429 |
| Total number of observations | 402521 | 3122 | 20048 |
| Number of reflections | 81686 | 575 | 4003 |
| <I/σ(I)> | 11.1 | 30.9 | 1.6 |
| Completeness [%] | 99.2 | 98.9 | 99.8 |
| Redundancy | 4.9 | 5.4 | 5 |
| CC(1/2) | 0.998 | 0.997 | 0.640 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.1 M Tris pH 8.0, 30% PEG 2,000 |
