6MEE
Crystal structure of broadly neutralizing antibody HEPC74
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.130, 63.586, 73.760 |
| Unit cell angles | 80.31, 74.11, 83.73 |
Refinement procedure
| Resolution | 35.097 - 1.360 |
| R-factor | 0.1684 |
| Rwork | 0.167 |
| R-free | 0.19060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mwf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.063 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.130 | 39.130 | 1.380 |
| High resolution limit [Å] | 1.360 | 7.450 | 1.360 |
| Rmerge | 0.068 | 0.042 | 1.370 |
| Rmeas | 0.074 | 0.044 | 1.495 |
| Rpim | 0.028 | 0.016 | 0.587 |
| Total number of observations | 1209314 | 9526 | 58622 |
| Number of reflections | 190823 | 1214 | 9351 |
| <I/σ(I)> | 11.5 | 38.8 | 1.3 |
| Completeness [%] | 93.7 | 98.4 | 92.1 |
| Redundancy | 6.3 | 7.8 | 6.3 |
| CC(1/2) | 0.999 | 0.999 | 0.451 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.2 M ammonium acetate, 0.1M BIS-Tris pH5.5, and 25% PEG 3,350 |
