6MDZ
Human Argonaute2-miR-122 bound to a target RNA with two central mismatches (bu2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979500 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 107.615, 138.289, 152.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.293 - 3.400 |
R-factor | 0.2219 |
Rwork | 0.220 |
R-free | 0.26430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4w5o |
RMSD bond length | 0.002 |
RMSD bond angle | 0.571 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.500 | 3.580 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.166 | 0.564 |
Rpim | 0.080 | 0.268 |
Number of reflections | 31940 | 4579 |
<I/σ(I)> | 8.4 | 3 |
Completeness [%] | 99.5 | 99 |
Redundancy | 5.3 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 10% PEG3350, 50mM Tris pH8, 20mM MgCl2, 75mM Phenol |