6MDX

Mechanism of protease dependent DPC repair

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	128.324, 29.507, 50.298
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.830 - 1.550
R-factor	0.16145
Rwork	0.159
R-free	0.19278
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.660
Data scaling software	HKL-3000
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.580
High resolution limit [Å]	1.550	1.550
Rpim	0.028	0.510
Number of reflections	28797	1396
<I/σ(I)>	29.8	2.2
Completeness [%]	99.8	99.8
Redundancy	13.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1 M sodium citrate tribasic dihydrate at pH 5.5 and 14% (w/v) PEG8000