6MDE
Mevalonate kinase from Methanosarcina mazei with mevalonate bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-30 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.050, 59.330, 191.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.662 - 2.100 |
R-factor | 0.1982 |
Rwork | 0.196 |
R-free | 0.24960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hac |
RMSD bond length | 0.008 |
RMSD bond angle | 0.883 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.662 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.111 | |
Number of reflections | 36591 | |
<I/σ(I)> | 9.97 | 2.1 |
Completeness [%] | 99.1 | 99.39 |
Redundancy | 3.5 | 3.5 |
CC(1/2) | 0.999 | 0.866 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 100 mM Bis-Tris, pH 5.5, 200-300 mM sodium potassium tartrate, 15-25% PEG8000 |