6MCY
Crystal structure of mouse Bak
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.951, 59.261, 79.207 |
| Unit cell angles | 93.14, 93.35, 97.63 |
Refinement procedure
| Resolution | 36.543 - 1.748 |
| R-factor | 0.182 |
| Rwork | 0.181 |
| R-free | 0.21780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2imt |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.750 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.540 | 1.811 |
| High resolution limit [Å] | 1.748 | 1.748 |
| Rmerge | 0.110 | |
| Rpim | 0.065 | |
| Number of reflections | 65510 | 6395 |
| <I/σ(I)> | 9.08 | 0.72 |
| Completeness [%] | 97.5 | 95.24 |
| Redundancy | 3.9 | |
| CC(1/2) | 0.997 | 0.326 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 281 | 20% PEG 3350, 200 mM ammonium formate |
