6MCU
Crystal structure of the holo retinal-bound domain-swapped dimer Q108K:T51D:A28H mutant of human Cellular Retinol Binding Protein II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-07-20 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.874, 73.992, 349.989 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.201 - 2.572 |
R-factor | 0.2334 |
Rwork | 0.231 |
R-free | 0.29630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rct |
RMSD bond length | 0.003 |
RMSD bond angle | 0.540 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.201 | 2.664 |
High resolution limit [Å] | 2.572 | 2.572 |
Rmerge | 0.690 | |
Rmeas | 0.150 | 0.796 |
Rpim | 0.311 | |
Number of reflections | 50840 | 2456 |
<I/σ(I)> | 12.08 | |
Completeness [%] | 91.3 | |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | PEG 4000, ammonium sulfate, sodium acetate |