6MCT
A designed pentameric membrane protein stabilized by van der Waals interaction
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-18 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.11583 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 52.894, 165.630, 41.067 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.387 - 1.900 |
R-factor | 0.1805 |
Rwork | 0.179 |
R-free | 0.21350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | designed |
RMSD bond length | 0.016 |
RMSD bond angle | 1.229 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.000 | 2.010 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.108 | 1.384 |
Rmeas | 0.113 | 1.440 |
Number of reflections | 29360 | 2896 |
<I/σ(I)> | 14.49 | 1.75 |
Completeness [%] | 99.6 | 99.8 |
Redundancy | 12.6 | 12.3 |
CC(1/2) | 1.000 | 0.718 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 5 mg/ml protein in 45 mM C8E4 mixed (1:1) with 2.5M ammonium sulfate, 50 mM Hepes pH 7.5. |