6M9R
Crystal structure of SznF from Streptomyces achromogenes var. streptozoticus NRRL 2697 with a bound N(delta)-hydroxy-N(omega)-methyl-L-arginine intermediate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-02-09 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.950, 107.730, 150.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.080 - 1.690 |
R-factor | 0.17121 |
Rwork | 0.170 |
R-free | 0.19076 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6m9s |
RMSD bond length | 0.007 |
RMSD bond angle | 1.172 |
Data reduction software | XDS (VERSION Jan 26, 2018) |
Data scaling software | XSCALE (VERSION Jan 26, 2018) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.090 | 1.790 |
High resolution limit [Å] | 1.685 | 1.690 |
Rmerge | 0.084 | 0.877 |
Rmeas | 0.089 | 0.935 |
Number of reflections | 107050 | |
<I/σ(I)> | 14.01 | 2.52 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 8.2 | 8.2 |
CC(1/2) | 0.998 | 0.825 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1 M Tris, pH 8.5, 0.1 M magnesium chloride, 14-20% w/v PEG4000 |