6M84
Crystal structure of cKir2.2 force open mutant in complex with PI(4,5)P2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-02-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | I 4 |
| Unit cell lengths | 82.787, 82.787, 182.976 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.810 |
| R-factor | 0.224 |
| Rwork | 0.222 |
| R-free | 0.26930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kuk |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.026 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
| Rmerge | 0.059 | 0.041 | 0.947 |
| Rmeas | 0.064 | 0.045 | 1.029 |
| Rpim | 0.025 | 0.017 | 0.401 |
| Total number of observations | 100653 | ||
| Number of reflections | 15052 | 1529 | 1490 |
| <I/σ(I)> | 11.7 | ||
| Completeness [%] | 99.9 | 99.7 | 99.9 |
| Redundancy | 6.7 | 6.6 | 6.5 |
| CC(1/2) | 0.994 | 0.708 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.4 | 298 | PEG400, trisodium citrate, tris |
