6M7P

Human DNA polymerase eta extension complex with cdA at the -2 position

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	98.495, 98.495, 81.708
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.990 - 1.750
R-factor	0.1839
Rwork	0.181
R-free	0.21760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ecv
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_282))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.780
High resolution limit [Å]	1.750	4.740	1.750
Rmerge	0.050	0.023	0.636
Total number of observations	158326
Number of reflections	45429	2325	2234
<I/σ(I)>	15.5
Completeness [%]	99.9	99.4	99.7
Redundancy	3.5	3.7	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	16 % PEG 2000 MME 100 mM MES, pH 6.0