6M7A

Structure of REV7-R124A complexed with SHLD3(28-73)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-07
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	56.521, 62.124, 130.471
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.900
Rwork	0.180
R-free	0.23240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vu7
RMSD bond length	0.009
RMSD bond angle	1.019
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.16-3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.970
High resolution limit [Å]	1.900	1.900
Number of reflections	37019	3640
<I/σ(I)>	2.4
Completeness [%]	100.0
Redundancy	11.2
CC(1/2)	0.433	0.450

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M NaCl Sodium Chloride; 0.1 M Na/K phosphate 6.2; 20% PEG1000