6M71
SARS-Cov-2 RNA-dependent RNA polymerase in complex with cofactors
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK II |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Energy Filter | GIF Bioquantum |
Phase Plate | VOLTA PHASE PLATE |
Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.9 |
Number of Particle | 2334248 |
Software | cryoSPARC,SerialEM,UCSF Chimera,PHENIX |
Symmetry | C1 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |