6M6S

Crystal structure of Caenorhabditis elegans Dicer-related helicase 3 (DRH-3) C-terminal domain with 5'-ppp 12-mer dsRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 2 21 21
Unit cell lengths	33.775, 93.090, 133.823
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.030 - 1.600
R-factor	0.1707
Rwork	0.169
R-free	0.19880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m6r
RMSD bond length	0.017
RMSD bond angle	1.881
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (dev_1137)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.960	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.042	0.761
Rmeas	0.064	1.021
Number of reflections	56358	2845
<I/σ(I)>	14.9
Completeness [%]	99.4	99.9
Redundancy	3.1	3.1
CC(1/2)		0.586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291.15	0.1 M Tris pH 7.5, 30% w/v polyethylene glycol 6000