6M6S
Crystal structure of Caenorhabditis elegans Dicer-related helicase 3 (DRH-3) C-terminal domain with 5'-ppp 12-mer dsRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 33.775, 93.090, 133.823 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.030 - 1.600 |
R-factor | 0.1707 |
Rwork | 0.169 |
R-free | 0.19880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6m6r |
RMSD bond length | 0.017 |
RMSD bond angle | 1.881 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1137) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.960 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.042 | 0.761 |
Rmeas | 0.064 | 1.021 |
Number of reflections | 56358 | 2845 |
<I/σ(I)> | 14.9 | |
Completeness [%] | 99.4 | 99.9 |
Redundancy | 3.1 | 3.1 |
CC(1/2) | 0.586 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | 0.1 M Tris pH 7.5, 30% w/v polyethylene glycol 6000 |