6M3R
Crystal structure of AnkG/beta4-spectrin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 139.591, 139.591, 211.317 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.896 - 4.313 |
| R-factor | 0.2583 |
| Rwork | 0.256 |
| R-free | 0.29520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d8o 3kbt |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.310 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 4.370 |
| High resolution limit [Å] | 4.300 | 11.630 | 4.300 |
| Rmerge | 0.159 | 0.053 | |
| Rmeas | 0.142 | 0.054 | |
| Rpim | 0.059 | 0.013 | 0.602 |
| Total number of observations | 259971 | ||
| Number of reflections | 16538 | 914 | 805 |
| <I/σ(I)> | 2.2 | ||
| Completeness [%] | 99.9 | 99.5 | 100 |
| Redundancy | 15.7 | 14.8 | 16.1 |
| CC(1/2) | 0.999 | 0.510 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 289 | 0.8 M potassium sodium tartrate tetrahydrate, 0.1 M Tris, pH 8.5 and 0.5% w/v polyethylene glycol monomethyl ether 5,000 |
