6M3B

hAPC-c25k23 Fab complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	112.277, 94.889, 114.320
Unit cell angles	90.00, 105.49, 90.00

Refinement procedure

Resolution	27.542 - 2.200
R-factor	0.2198
Rwork	0.218
R-free	0.24750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1aut
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.260
High resolution limit [Å]	2.200	2.200
Rmerge	0.096	0.989
Rpim		0.531
Number of reflections	57099	4288
<I/σ(I)>	32.22	3.04
Completeness [%]	97.2
Redundancy	4.2
CC(1/2)	0.991	0.825

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1% n-Octyl-beta-D-glucoside, 0.1M sodium citrate tribasic dehydrate pH 5.5, 22% (w/v) PEG 3350