6M3B
hAPC-c25k23 Fab complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 112.277, 94.889, 114.320 |
Unit cell angles | 90.00, 105.49, 90.00 |
Refinement procedure
Resolution | 27.542 - 2.200 |
R-factor | 0.2198 |
Rwork | 0.218 |
R-free | 0.24750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1aut |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.096 | 0.989 |
Rpim | 0.531 | |
Number of reflections | 57099 | 4288 |
<I/σ(I)> | 32.22 | 3.04 |
Completeness [%] | 97.2 | |
Redundancy | 4.2 | |
CC(1/2) | 0.991 | 0.825 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1% n-Octyl-beta-D-glucoside, 0.1M sodium citrate tribasic dehydrate pH 5.5, 22% (w/v) PEG 3350 |