6M2V
Crystal structure of UHRF1 SRA complexed with fully-mCHG DNA.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-08-30 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.897, 74.829, 141.505 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.980 - 3.000 |
| R-factor | 0.2363 |
| Rwork | 0.234 |
| R-free | 0.27610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zo0 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.708 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP (11.7.02) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.980 | 3.107 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.175 | |
| Rmeas | 0.189 | |
| Rpim | 0.070 | |
| Number of reflections | 12430 | 1240 |
| <I/σ(I)> | 9.24 | |
| Completeness [%] | 99.7 | 99.92 |
| Redundancy | 7.2 | 7.3 |
| CC(1/2) | 0.997 | 0.764 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.1M Bis-Tris, pH 8.0, 0.2M NaCl, 12% PEG 3,350 |
