6M2C
Distinct mechanism of MUL1-RING domain simultaneously recruiting E2 enzyme and the substrate p53-TAD domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2016-05-04 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.207, 141.241, 68.047 |
Unit cell angles | 90.00, 104.81, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 5.810 | 2.700 |
Rmerge | 0.085 | 0.054 | 0.287 |
Rmeas | 0.093 | 0.059 | 0.337 |
Rpim | 0.038 | 0.022 | 0.171 |
Total number of observations | 107188 | ||
Number of reflections | 21200 | 2388 | 1768 |
<I/σ(I)> | 13.2 | ||
Completeness [%] | 89.9 | 99.3 | 74.8 |
Redundancy | 5.1 | 6.9 | 3 |
CC(1/2) | 0.998 | 0.618 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 4% v/v Tacsimate pH 6.0, 12% w/v Polyethylene glycol 3350 |