6M1U
Crystal structure of the vertebrate conserved region (VCR) of human METTL16
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | H 3 2 |
Unit cell lengths | 139.610, 139.610, 53.130 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.641 - 2.791 |
R-factor | 0.2349 |
Rwork | 0.234 |
R-free | 0.26300 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.641 | 48.641 | 2.860 |
High resolution limit [Å] | 2.790 | 12.480 | 2.790 |
Rmerge | 0.343 | 0.065 | 4.761 |
Rmeas | 0.346 | 0.065 | 4.791 |
Total number of observations | 410237 | ||
Number of reflections | 5022 | 65 | 356 |
<I/σ(I)> | 17.68 | 66.17 | 1.26 |
Completeness [%] | 99.8 | 97 | 98.1 |
Redundancy | 81.688 | 64.892 | 81.792 |
CC(1/2) | 0.999 | 0.999 | 0.634 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM Tris-HCl, pH 7.4, 20% (w/v) PEG3350, 200 mM sodium citrate, 4% (w/v) formamide and 5 mM dextran sulfate sodium salt. |