6M0V
Crsytal structure of streptococcus thermophilus Cas9 in complex with the GGAA PAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 200 |
| Detector technology | PIXEL |
| Collection date | 2019-02-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 321.839, 75.078, 70.109 |
| Unit cell angles | 90.00, 93.21, 90.00 |
Refinement procedure
| Resolution | 48.822 - 3.000 |
| R-factor | 0.2156 |
| Rwork | 0.213 |
| R-free | 0.25660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5axw |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.647 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 31477 | 1824 |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.6 | |
| CC(1/2) | 0.800 | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | KCL, MgCl2, MPD, MgCl2 |
