6LZO
Thermolysin with 1,10-phenanthroline
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-05-27 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 92.772, 92.772, 129.103 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.850 - 1.800 |
R-factor | 0.1746 |
Rwork | 0.172 |
R-free | 0.21070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ig7 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.553 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.086 | 0.377 |
Rmeas | 0.090 | 0.405 |
Rpim | 0.024 | 0.142 |
Number of reflections | 30705 | 1490 |
<I/σ(I)> | 34.5 | 4.233 |
Completeness [%] | 99.0 | 98.7 |
Redundancy | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294.15 | Tris-HCl, glycerol, Ammonium sulfate |