6LZL
Crystal structure of human dihydroorotate dehydrogenase (DHODH) with Piperine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2016-03-08 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.987 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 90.413, 90.413, 122.699 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.290 - 1.980 |
R-factor | 0.1863 |
Rwork | 0.185 |
R-free | 0.20930 |
Structure solution method | AB INITIO PHASING |
Data reduction software | CrysalisPro (1.0) |
Data scaling software | HKL-3000 (2.0) |
Phasing software | PHENIX (7.0) |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.290 | 1.980 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.313 | 0.313 |
Number of reflections | 38437 | |
<I/σ(I)> | 5.45 | |
Completeness [%] | 98.8 | |
Redundancy | 14.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | HEPES pH 7.7, NaCl, 20% Glycerol, EDTA, UDAO, DDAO |