6LYV

The crystal structure of SAUGI/KSHVUDG complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97622
Spacegroup name	P 1
Unit cell lengths	85.887, 92.196, 98.107
Unit cell angles	108.14, 90.61, 92.23

Refinement procedure

Resolution	19.960 - 2.700
R-factor	0.1938
Rwork	0.192
R-free	0.23150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ays
RMSD bond length	0.003
RMSD bond angle	0.597
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.096	0.437
Number of reflections	74977	7580
<I/σ(I)>	7.5
Completeness [%]	96.2
Redundancy	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	tri-Ammonium citrate, PEG 3350