6LYL
Crystal structure of S1052D mutant of Formylglycinamidine synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-11-30 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5417 |
| Spacegroup name | P 65 |
| Unit cell lengths | 146.193, 146.193, 141.053 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.850 - 2.100 |
| R-factor | 0.1546 |
| Rwork | 0.153 |
| R-free | 0.19190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t3t |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.927 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmeas | 0.107 | |
| Rpim | 0.267 | |
| Number of reflections | 99120 | 4898 |
| <I/σ(I)> | 15.9 | |
| Completeness [%] | 99.5 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 2M Ammonium Sulphate |
